Extraction And Bioevaluation Of Nigella Sativa Meal Protein Isolates In Relation To Protein Enriched Cookies
作者： Rizwana Batool
DOI:
关键词: Meal 、 Net protein utilization 、 Protein efficiency ratio 、 Nigella sativa 、 Food science 、 Nitrogen balance 、 Context (language use) 、 Biological value 、 Biochemistry 、 Isoelectric point 、 Chemistry
摘要: Nigella sativa meal is a byproduct obtained after oil extraction that contains appreciable amount of protein.In the current exploration, characterization, and bioevaluation protein isolates were conducted to develop enriched cookies.The prepared through different methods i.e. isoelectric precipitation, micellization enzymatic extraction.The highest yield 79.69i?½0.70% was estimated in (EPI) followed by 64.65i?½0.64% precipitation (IPI) whilst, lowest output 46.40i?½0.47% noticed micellized (MPI).Furthermore, resultant evaluated for their functional properties.In this context, bulk density recorded EPI IPI MPI.Conversely, maximum water absorption capacity observed trailed MPI nonetheless, higher EPI.The foaming (FC) MPI. Likewise, emulsion (60.74i?½ 0.61%) SDS-PAGE depicted had bands range 6.5 116 kDa.Quantification essential non-essential amino acid also carried out with reference profile.Amongst acids, lysine content (38.75i?½0.38 mg/100g) (34.04i?½0.34 concentration (29.33i?½0.29 mg/100g).The scores 65.43, 53.27 & 55.14 EPI, MPI, respectively.Additionally, PDCAAS 82.25i?½1.56% 80.21i?½1.13% whereas, minimum 73.75i?½1.54% during session 2010-11.Afterwards, trial growth nitrogen balance study parameters.The efficiency ratio (PER), net (NPR) relative (RNPR) respectively year 2010-11.Similarly, parameters expounded true digestibility (TD), biological value (BV) utilization (NPU) IPI.Similar trend studies 2nd year. On basis overall yield, properties bioevaluation, one best isolate namely selected cookies preparation.For purpose, composite blends formulated analyzed chemical rheological mixographic farinographic characteristics.Afterwards, physicochemical characteristics, profile hedonic response.On compiled data these traits, it concluded (T3) 15% supplementation enzymatically extracted performed better has potential address menace energy malnutrition.
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