Generation and detection of Z-DNA.

摘要: Publisher Summary Z-DNA is the only known helical conformation of DNA that left-handed. The helix longer and thinner than B-DNA, bases lie relatively farther from axis, creating a single deep, narrow groove. This chapter discusses techniques available for inducing formation detecting it. left-handed sense means any torsional stress directed opposite right-handed B-DNA will tend to favor Z-DNA. syn-anti alternation dictates alternating purine–pyrimidine sequences most easily form because pyrimidines anti orientation, whereas purines can accommodate either syn or conformation. Because displacement be stabilized by adding bulky groups at sites are sterically hindered in but become more accessible Z Similarly, certain chemicals react those used recognize overall conformational differences between permit two structures distinguished many nucleases DNA-binding proteins, which generally DNA, antibodies specifically against