Low Concentration Protein Detection Using Novel SERS Devices

作者: Francesco De Angelis , Patrizio Candeloro , Maria Laura Coluccio , Carlo Liberale , G. Cojoc

DOI: 10.1007/978-3-642-15175-0_12

关键词: Rhodamine 6GSurface plasmonRaman spectroscopyAtmospheric temperature rangeAnalytical chemistryMyoglobinNanolithographyMoleculeMaterials scienceRaman scattering

摘要: We report, herein, novel processes of nanofabrication techniques for few molecules detection by generating surface plasmons, thus giving a giant electric field in controllable and reproducible manner. Surface enhanced Raman scattering (SERS) measurements are performed different proteins, namely, lysozyme, ribonuclease-B, bovin serum albumin, ferritin, myoglobin the temperature range between –65°C 90°C, using “Device1”, which is fabricated means e-beam electro-plating technique. The calculation shows possible concentration down to attomole. in-depth analysis even small conformational changes 2D correlation difference order gain straightforward understanding proteins undergoing thermodynamical perturbation. “Device2” metal electroless investigate Rhodamine 6G (R6G) concentrations. Modifying this device bimetal (Ag Au) deposition permits us further enhancement signal better durability.

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