Evidence for a multidrug resistance-associated protein 1 (MRP1)-related transport system in cultured rat liver biliary epithelial cells
作者： Arnaud Courtois , Léa Payen , Dominique Lagadic , André Guillouzo , Olivier Fardel
DOI: 10.1016/S0024-3205(98)00618-3
关键词: Multidrug Resistance-Associated Proteins 、 Cell biology 、 Biology 、 Multidrug Resistance-Associated Protein 1 、 Efflux 、 Membrane transport 、 Dichlorofluorescein 、 ATP-binding cassette transporter 、 Organic anion 、 Probenecid 、 General Pharmacology, Toxicology and Pharmaceutics 、 General Biochemistry, Genetics and Molecular Biology 、 General Medicine
摘要: Cellular accumulation and efflux of the anionic fluorescent dye carboxy-2',7'-dichlorofluorescein (CF) were studied in rat liver SDVI cells thought to derive from primitive bile ductules, order characterize carrier-related membrane transport organic anions epithelial cells. Probenecid, a common blocker anion transport, was found strongly enhance CF levels dose-dependent manner through inhibition efflux. Such an outwardly-directed demonstrated be temperature-dependent down-regulated by various metabolic inhibitors, therefore outlining its requirement for energy; it shown Na+- potential-independent inhibited drugs such as indomethacin, indoprofen rifamycin B. These functional features are closed those described multidrug resistance-associated protein 1 (MRP1) that furthermore demonstrated, contrast P-glycoprotein, expressed lower MRP1-overexpressing drug-resistant tumor data suggest active occurs like cultured biliary MRP1-related system.
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