High molecular weight DNA from fixed cytogenetic preparations.

摘要: Cell pellets that have been stored in routine clinical cytogenetic fixative were studied for the presence of intact DNA. A method isolation high molecular weight DNA from fixed preparations human leukocytes, bone marrow, and cell hybrid cultures is presented. cleaved with restriction enzymes, transferred to nitrocellulose filters after agarose gel electrophoresis, hybridized radiolabeled probes demonstrate could yield sufficient quality Southern blot hybridization analysis. This protocol may be useful analysis patients who are unavailable additional sampling.