The role of nonbilayer lipid structures in the fusion of human erythrocytes induced by lipid fusogens
作者： M.J. Hope , P.R. Cullis
DOI: 10.1016/0005-2736(81)90533-2
关键词: Bilayer 、 Biochemistry 、 Chemistry 、 Phase (matter) 、 Biophysics 、 Micelle 、 Conformational change 、 Mechanism of action 、 Membrane 、 Lipid bilayer fusion 、 Membrane lipids
摘要: Abstract 1. (1) A comparative study of the polymorphic phase behaviour reconstituted erythrocyte phospholipids with ‘fusogens’ and chemically related ‘non-fusogens’ using 31P-NMR techniques has been made. We demonstrate that only fusogens can induce a conformational change in membrane lipids from bilayer to hexagonal (HII) phase. 2. (2) The tendency (ghost) membranes adopt nonbilayer structures is correlated extent fusion erythrocytes at various concentrations glycerol monooleate. Above certain critical concentration monooleate, there an increase ghost HII phase, which paralleled by marked processes intact erythrocytes. 3. (3) These observations are consistent common mechanism action for fusogenic whereby inverted lipid such as long cylinders (HII configuration) or micelles induced, serve intermediates event.
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