Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation.

摘要: Environmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations premature aging. DNA damage its faulty repair lead to cell death promote cancer through the accumulation of mutations. Misrepair germ cells is particularly dangerous as it may alterations developmental programs genetic disease offspring. pathways radical defense mechanisms mediate resistance genotoxic stresses. Here, we investigated, fission yeast Schizosaccharomyces pombe, role H2O2-detoxifying enzyme cytosolic catalase T (Ctt1) Fe2+/Mn2+ symporter Pcl1 protecting meiotic chromosome dynamics gamete formation from radicals generated by ROS IR. We found that wild-type pcl1-deficient respond similarly X ray doses up 300 Gy, while ctt1∆ meiocytes showed a moderate sensitivity IR but hypersensitivity hydrogen peroxide with dying at >0.4 mM H2O2. Meiocytes deficient for pcl1, on other hand, similar wild type, surviving >40 mM. In all, appears absence main pathway S. pombe are able survive significant IR-induced radicals.