Positive contrast from cells labeled with iron oxide nanoparticles: Quantitation of imaging data

摘要: Author(s): Magnitsky, Sergey; Zhang, Jinjin; Idiyatullin, Djaudat; Mohan, Geetha; Garwood, Michael; Lane, Nancy E; Majumdar, Sharmila | Abstract: PurposeConventional T2 -weighted MRI produces a hypointense signal from iron-labeled cells, which renders quantification unfeasible. We tested SWeep Imaging with Fourier Transformation (SWIFT) pulse sequence to generate quantifiable hyperintense cells.MethodsMesenchymal stem cells (MSCs) were labeled different concentrations of iron oxide particles and examined for cell viability, proliferation, differentiation. The SWIFT was optimized detect quantify the amount in muscle tissue after injection solution MSCs.ResultsThe incubation MSCs low concentration poly-L-lysine mixture resulted an internalization up 22 pg per no adverse effect on MSCs. Phantom experiments showed dependence intensity excitation flip angle. or solutions detected, quantified variable angle method.ConclusionsThe can produce cells. graft 18 x 106 detectable 19 days quantifiable. proposed protocol simplifies detection provides means numbers. Magn Reson Med 78:1900-1910, 2017. © 2017 International Society Magnetic Resonance Medicine.