[103a] The use of guanidinium chloride in the isolation of nucleic acids

摘要: Publisher Summary This chapter discusses and investigates the use of guanidinium chloride in isolation nucleic acids explains that nucleoprotein is dissociated into RNA protein by 4 M-guanidinium chloride, which also inhibits action ribonuclease. precipitated from solution cold, acidification to pH 5 at 0° or addition alcohol (0.5 volume) 0°. The precipitate purified redissolution in, precipitation from, chloride. In a preliminary experiment, sedimentation pattern component ribosomes (100 μg/ml) (4 M) obtained means an analytical ultracentrifuge fitted with ultraviolet optics establish undegraded. omission purification procedure leads contaminated one ribosome suspension was added divided two parts. Alcohol each RNA. precipitates were separated low speed centrifuging. case, dissolved ultimately product had pattern. other immediately extracted water and, once dissolution achieved 0°, aliquot removed, bentonite inhibit ribonuclease, obtained. M for (principally DNA) nuclear fraction described chapter, acid twice more. redissolved 25° dialyzed overnight against standard saline citrate solution. DNA may then be freed methods.