NMR of Protein-Lipid Interactions in Model and Biological Membrane Systems

摘要: Membranes are composed predominantly of lipids, proteins, and sterol molecules responsible at least in part for a wide variety biochemical processes such as respiration, vision, photosynthesis, cell-cell recognition, nerve impulse transmission. Not surprisingly then, there have been considerable efforts spent attempting to characterize the molecular structure of, intermolecular interactions between, individual membrane components, an attempt relate structures membranes their function. In this short review we discuss recent developments our understanding obtained by means NMR spectroscopic techniques. We show that protein-lipid both model intact biological characterized dynamic disordering boundary-lipid hydrocarbon chains, viewed high-field deuterium spectroscopy, while 31P spectra indicate significant and/or immobilization within phospholipid polar head group region due association with protein. The effects very different from those seen cholesterol, marked contrast old ideas rigid, ordered “boundary-lipid” surrounding proteins. also present results suggesting it will soon be possible directly monitor lipid on protein dynamics spectroscopy.