作者: A. Nakorchevsky , J.R. Yates
DOI: 10.1016/B978-0-12-374920-8.00121-1
关键词: Mass spectrometry 、 Protein tertiary structure 、 Biomolecular structure 、 Characterization (materials science) 、 Functional protein 、 Biophysics 、 Chemistry 、 Functional proteomics 、 Proteomics 、 Computational biology
摘要: This chapter discusses the application of mass spectrometry to functional and structural characterization proteins. Mass techniques commonly applied both proteomics (identity expression level proteins) proteins (post-translational modifications, tertiary structure stability, subunit composition, molecular shape) are discussed. The reviews current historical methodologies for these applications, provides an in-depth description instrumentation requirements, highlights some examples used. Overall, biophysical, biochemical, analytical, biological aspects protein spectrometry.