Detection and Quantification of Early-Stage Malaria Parasites in Laboratory Infected Erythrocytes by Attenuated Total Reflectance Infrared Spectroscopy and Multivariate Analysis
作者： Aazam Khoshmanesh , Matthew W. A. Dixon , Shannon Kenny , Leann Tilley , Don McNaughton
DOI: 10.1021/AC500199X
关键词: Detection limit 、 Attenuated total reflection 、 Chemistry 、 Malaria 、 Plasmodium falciparum 、 Gametocyte 、 Multivariate analysis 、 Infrared spectroscopy 、 Virology 、 Parasitemia
摘要: New diagnostic modalities for malaria must have high sensitivity and be affordable to the developing world. We report on a method rapidly detect quantify different stages of parasites, including ring gametocyte forms, using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) partial least-squares regression (PLS). The absolute detection limit was found 0.00001% parasitemia (<1 parasite/μL blood; p < 0.008) cultured early stage parasites in suspension normal erythrocytes. Future development universal robust calibration models can significantly improve diagnoses, leading earlier treatment this devastating disease.
acs.org
sci-hub.se 参考文章(28)
Silvina Pagola, Peter W. Stephens, D. Scott Bohle, Andrew D. Kosar, Sara K. Madsen, The structure of malaria pigment β-haematin Nature. ,vol. 404, pp. 307- 310 ,(2000) , 10.1038/35005132
Katherine E. Jackson, Nectarios Klonis, David J. P. Ferguson, Akinola Adisa, Con Dogovski, Leann Tilley, Food vacuole-associated lipid bodies and heterogeneous lipid environments in the malaria parasite, Plasmodium falciparum Molecular Microbiology. ,vol. 54, pp. 109- 122 ,(2004) , 10.1111/J.1365-2958.2004.04284.X
M. Foley, L.W. Deady, K. Ng, A.F. Cowman, L. Tilley, Photoaffinity labeling of chloroquine-binding proteins in Plasmodium falciparum. Journal of Biological Chemistry. ,vol. 269, pp. 6955- 6961 ,(1994) , 10.1016/S0021-9258(17)37467-7
John M Pisciotta, Isabelle Coppens, Abhai K Tripathi, Peter F Scholl, Joel Shuman, Sunil Bajad, Vladimir Shulaev, David J Sullivan Jr, None, The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallization. Biochemical Journal. ,vol. 402, pp. 197- 204 ,(2007) , 10.1042/BJ20060986
Melvin A Ambele, Timothy J Egan, Neutral lipids associated with haemozoin mediate efficient and rapid β-haematin formation at physiological pH, temperature and ionic composition Malaria Journal. ,vol. 11, pp. 337- 337 ,(2012) , 10.1186/1475-2875-11-337
Noppadon Tangpukdee, Chatnapa Duangdee, Polrat Wilairatana, Srivicha Krudsood, Malaria diagnosis: a brief review. Korean Journal of Parasitology. ,vol. 47, pp. 93- 102 ,(2009) , 10.3347/KJP.2009.47.2.93
D. Szaller, B. G. Vértessy, B. G. Vértessy, T. Zelles, A. Bóta, V. Kocsis, I. Kézsmárki, A. Orbán, L. F. Kiss, S. Bordács, A. Butykai, E. Tátrai-Szekeres, Malaria pigment crystals as magnetic micro-rotors: key for high-sensitivity diagnosis Scientific Reports. ,vol. 3, pp. 1431- 1431 ,(2013) , 10.1038/SREP01431
Bayden R. Wood, Antje Hermelink, Peter Lasch, Keith R. Bambery, Grant T. Webster, Mehdi Asghari Khiavi, Brian M. Cooke, Samantha Deed, Dieter Naumann, Don McNaughton, Resonance Raman microscopy in combination with partial dark-field microscopy lights up a new path in malaria diagnostics Analyst. ,vol. 134, pp. 1119- 1125 ,(2009) , 10.1039/B822603B
Jenny Liu, Sepideh Modrek, Roly D Gosling, Richard GA Feachem, Malaria eradication: is it possible? Is it worth it? Should we do it? The Lancet Global Health. ,vol. 1, ,(2013) , 10.1016/S2214-109X(13)70002-0
Yi-Ren Liu, Ji-Chun Zhao, Patients in China face crisis The Lancet. ,vol. 381, ,(2013) , 10.1016/S0140-6736(13)60132-4