Plant Microtechnique and Microscopy

摘要: 1. Quick Start Paraffin Embedding - Microwave method Glycol methacrylate embedding Butyl/methyl Steedman's wax Chromosome squashes Hematoxylin staining microwave technique Kohler illumination 2. Microscopy History of the compound microscope Principles specimen 3. Chemical Fixation Tissues The quality fixation mechanics Coagulating Fixatives Crosslinking Agents Other methods Recommendations for fixing plant tissues 4. Tissue Dehydration General protocol using a graded dehydration series chemical agents 5. Infiltrating infiltrating and PEG Polyethylene glycol derivatives Plastic 6. Sectioning Mounting blocks to microtome stubs sectioning knife Setting up Methods sections glass slides 7. Staining Dye Chemistry equipment histological microtechniques stains protocols coverslip Coverslip mounting media 8. Alternate Microtomy Free-hand Sections Vibratome Cryostat Sliding 9. Special Clearing with NaOH chloral hydrate without removing cytoplasmic components Macerating woody non-woody Bleaching Preserving color in whole mounted specimens 10. Problem Solving collection Tannins Hard materials Dehydration, Infiltration, Hand 11. Histochemistry Cytochemistry relationship probe target Cytochemical localization cell enzymes Fluorescence 12. Localization Molecular Targets Immunolocalization Printing situ hybridization APPENDIX I: TOXICS II: BUFFERS III: COMMON CALCULATIONS IV: MERCURY ARC LAMP V: MANUFACTURERS AND VENDORS VI: OPTICS Properties Light lens optics objective eyepiece condensor microscopy light sources BIBLIOGRAPHY INDEX