Array comparative genomic hybridization and expression analyses for the identification of candidate oncogenes co-amplified on chromosomes 8p and 11q in breast cancer

摘要: Proc Amer Assoc Cancer Res, Volume 47, 2006 4190 Co-amplification of chromosomes 11q12-11q14 and 8p12-8p11 occurs frequently together in breast cancer. Preliminary analyses 60 ductal invasive tumors with whole genome array Comparative Genomic Hybridization (array CGH) show that 7% contain both these amplifications, another amplification on chromosome 11q but not 8p, 3% 8p 11q. To identify the minimal amplified regions 11q, CGH was carried out a high-resolution containing contig overlapping DNA sequences regions. Eight cell lines from tumors, three amplifications two five previously analyzed were array. Real-time PCR used to determine expression RNA candidate genes comparison without Affymetrix data further set 90 54 all forms aberrations narrow down Where antibodies are available, protein levels some correlate levels. Functional assays underway which driving oncogenes if there cooperating aberrations.