Elicitor-induced chemiluminescence in cell suspension cultures of tomato, sweet pepper and tobacco plants and its inhibition by suppressors from Phytophthora spp

作者: Leandro M. Sanchez , Noriyuki Doke , Kazuhito Kawakita

DOI: 10.1016/0168-9452(93)90085-E

关键词: BotanyBiologyElicitorSolanaceaeCatalaseNicotiana tabacumPhytophthoraTironMicrobiologyLycopersiconPepper

摘要: Abstract The hyphal wall components (HWC) from Phytopthora capsici, P. infestans and nicotianae var. nicotinae , which caused the hypersensitive cell death of suspension-cultured cells sweet pepper ( Capsicum frutescence L.), tomato Lycopersicon esculentum Mill.) tobacco Nicotiana tabacum enhanced luminol-mediated chemiluminescence in suspensio-cultured each plant within several minutes after treatment with HWC a species-nonspecific manner. HWC-induced was by incubation superoxide dismututase but significanyly inhibited pretreatment catalase or, O 2 − scavenger, Tiron. Similarly, Tirom or water-soluble glucans (WSG) respective species Phytophthora suppressed both chemilunescence specific only to its own host. These results suggested that spp. may produce suppressor-gluncans inhibit activation generation responsible for triggering Solanaceous host plants permit establishment compatible interaction.

参考文章(20)
Anne J. Anderson, Kim Rogers, Craig S. Tepper, Kris Blee, Jeff Cardon, Timing of molecular events following elicitor treatment of plant cells Physiological and Molecular Plant Pathology. ,vol. 38, pp. 1- 13 ,(1991) , 10.1016/S0885-5765(05)80139-0
Judith A. Glazener, Elizabeth W. Orlandi, Gary L. Harmon, C.Jacyn Baker, An improved method for monitoring active oxygen in bacteria-treated suspension cells using luminol-dependent chemiluminescence Physiological and Molecular Plant Pathology. ,vol. 39, pp. 123- 133 ,(1991) , 10.1016/0885-5765(91)90023-B
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X