The effect of full length and mature NAG-1 protein overexpression on cytotoxicity of celecoxib, tamoxifen and doxorubicin in HT1080.

摘要: Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is involved in inflammation, apoptosis/survival and tumorigenesis as well resistance to chemotherapy. NAG-1 protein synthesized pro-peptide, cleaved secreted mature protein. Regulation of not completely discovered increased level has been reported many cancers. The expression cancer cells could affect the progression tumor growth. In addition secretion full length forms can influence cell proliferation other cells. this study role on viability HT-1080 MCF-7 were evaluated, cytotoxicity celecoxib, indomethacin, tamoxifen doxorubicin HT1080 stably expressing also tested.Full was cloned from cDNA library HCT116 transfected Cells treated with different concentrations assessed by MTT assay. effect conditioned medium tested.The growth curves different. presence compared untransfected higher. inhibited 24 48 hrs.NAG-1 enhances drug tamoxifen, indomethacin HT1080. addition, condition inhibits Thus, induce inhibition non suggested a marker for effective chemotherapy progression.