Specificity Protein 1 Regulates Gene Expression Related to Fatty Acid Metabolism in Goat Mammary Epithelial Cells

摘要: Specificity protein 1 (SP1) is a ubiquitous transcription factor that plays an important role in controlling gene expression. Although mediating the function of various hormones, SP1 regulating milk fat formation remains unknown. To investigate sequence and expression information, as well its modulating lipid metabolism, we cloned from mammary gland Xinong Saanen dairy goat. The full-length cDNA 4376 bp including 103 5'UTR, 2358 ORF (HM_236311) 1915 3'UTR, which predicted to encode 786 amino acids polypeptide. Phylogenetic tree analysis showed goat has closest relationship with sheep, followed by bovines (bos taurus, odobenus ceratotherium), pig, primates (pongo, gorilla, macaca papio) murine (rattus mus), while furthest was canis otolemur. Expression predominant lungs, small intestine, muscle, spleen, subcutaneous fat. There were no significant level differences between tissues collected at lactation dry-off period. Overexpression epithelial cells (GMECs) led higher mRNA peroxisome proliferator-activated receptor-γ (PPARγ) lower liver X receptor α (LXRα) level, both crucial fatty acid correspondingly altered their downstream genes GMECs. These results further enhanced silencing SP1. findings suggest may play metabolism.