A photoconvertible fluorescent reporter to track chaperone-mediated autophagy
作者： Hiroshi Koga , Marta Martinez-Vicente , Fernando Macian , Vladislav V. Verkhusha , Ana Maria Cuervo
DOI: 10.1038/NCOMMS1393
关键词: Intracellular 、 Proteasome 、 Autophagy 、 Flow cytometry 、 Cell biology 、 Downregulation and upregulation 、 Biology 、 Chaperone-mediated autophagy 、 In vitro 、 Cell culture
摘要: … However, we can foresee particular conditions in which the use of this reporter will need additional controls. For example, when used in cellular contexts favouring protein aggregation, …
nature.com
harvard.edu
elsevier.com
sci-hub.se 参考文章(30)
Brian Storrie, Edward Amadden, Isolation of subcellular organelles. Methods in Enzymology. ,vol. 182, pp. 203- 225 ,(1990) , 10.1016/0076-6879(90)82018-W
Cynthia N. Perry, Shiori Kyoi, Nirmala Hariharan, Hiromitsu Takagi, Junichi Sadoshima, Roberta A. Gottlieb, Novel methods for measuring cardiac autophagy in vivo. Methods in Enzymology. ,vol. 453, pp. 325- 342 ,(2009) , 10.1016/S0076-6879(08)04016-0
Antonia Follenzi, Luigi Naldini, Generation of HIV-1 derived lentiviral vectors. Methods in Enzymology. ,vol. 346, pp. 454- 465 ,(2002) , 10.1016/S0076-6879(02)46071-5
S S Wing, H L Chiang, A L Goldberg, J F Dice, Proteins containing peptide sequences related to Lys-Phe-Glu-Arg-Gln are selectively depleted in liver and heart, but not skeletal muscle, of fasted rats. Biochemical Journal. ,vol. 275, pp. 165- 169 ,(1991) , 10.1042/BJ2750165
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A. M. Cuervo, E. Knecht, S. R. Terlecky, J. F. Dice, Activation of a selective pathway of lysosomal proteolysis in rat liver by prolonged starvation. American Journal of Physiology-cell Physiology. ,vol. 269, ,(1995) , 10.1152/AJPCELL.1995.269.5.C1200
Ana Maria Cuervo, Chaperone-mediated autophagy: selectivity pays off Trends in Endocrinology and Metabolism. ,vol. 21, pp. 142- 150 ,(2010) , 10.1016/J.TEM.2009.10.003
A. M. Cuervo, J. F. Dice, A receptor for the selective uptake and degradation of proteins by lysosomes Science. ,vol. 273, pp. 501- 503 ,(1996) , 10.1126/SCIENCE.273.5274.501
J. M. Backer, J. F. Dice, Covalent linkage of ribonuclease S-peptide to microinjected proteins causes their intracellular degradation to be enhanced during serum withdrawal. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 5830- 5834 ,(1986) , 10.1073/PNAS.83.16.5830
Geert Hamer, Olli Matilainen, Carina I Holmberg, A photoconvertible reporter of the ubiquitin-proteasome system in vivo. Nature Methods. ,vol. 7, pp. 473- 478 ,(2010) , 10.1038/NMETH.1460