Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation

作者: Nurul Amirah Hassan , Sugenendran Supramani , Mohamad Nor Azzimi Sohedein , Siti Rokhiyah Ahmad Usuldin , Anita Klaus

DOI: 10.1016/J.BCAB.2019.101305

关键词: Food scienceGanoderma lucidumStipe (botany)Response surface methodologyChemistryMyceliumStrain (chemistry)Central composite designFermentationBiomass

摘要: Abstract A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap woody stipe. Molecularly, GLSB (642.8 bp) sequenced found to be 99% similar the Serbian-originated G. BEOFB 434 431. The isolate belongs species as sequence dissimilarities (Knuc) value between both sequences of same fungal 0.001. In submerged-liquid fermentation, biomass exopolysaccharide (EPS) production optimised using response surface methodology. interactions three variables: initial pH (4–6), temperature (20°C–30 °C), glucose concentration (10 g/L–50 g/L) were analysed a central composite design. An analysis variance revealed that model significant for all parameters investigated (p

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