Nitric oxide is an essential mediator for neuronal differentiation of rat primary cortical neuron cells.
作者： Soo-Jin Oh , Jee-In Heo , Yoon-Jung Kho , Jeong-Hyeon Kim , Hong-Joon Kang
关键词: Neuron 、 Nitric oxide 、 Neurite 、 Biology 、 Butyrate 、 Neuroscience 、 Cell biology 、 Nitric oxide synthase 、 Messenger RNA 、 Embryonic stem cell 、 Mediator
摘要: Nitric oxide (NO) regulates proliferation, differentiation and survival of neurons. Although NO is reported to involve in NGF-induced PC12 cells, the role has not been characterized primary neuron cells. Therefore, we investigated neuronal cortical Primary cells were prepared from rat embryos embryonic day 18 treated with NMMA (NOS inhibitor) or PTIO (NO scavenger). Neurite outgrowth was counted mRNA levels p21, p27, c-jun c-myc measured by RT-PCR. inhibited a little NOS inhibitor completely scavenger. The p21 differentiation-induced growth arrest genes increased during differentiation, but they decreased On other hand, level changed differently previously reported. these reversed inhibitor- scavenger-treated nNOS protein activity largely These results suggest that an essential mediator for
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