Use of Analogues and the Substrate-Sensitivity of Mutants in Analysis of Purine Uptake and Breakdown in Aspergillus nidulans

摘要: Aspergillus mutants resistant to various purine analogues (purine, 8-azaguanine, 2-thioxanthine, and 2-thiouric acid) are defective in at least one step of uptake or breakdown. The properties these show that there two systems for purines, which mediates the hypoxanthine, guanine, adenine, other, xanthine uric acid. Allantoinase-less strains sensitive toxic effects allantoin accumulation. They severely inhibited when grown presence naturally occurring purines. Mutant derived from these, may be isolated. These either wild-type revertants, carry a second metabolic block breakdown double confirm interpretation nature analogue-resistant mutants.