Automated detection of action potentials in interferometric imaging of spiking cells (Conference Presentation)

摘要: Wide-field interferometric imaging systems can detect mechanical deformations of a cell during an action potential (AP), such as in quantitative phase microscopy, which is highly sensitive to the changing optical path length. This enables non-invasive optophysiology spiking cells without exogeneous markers, but high-fidelity requires averaging large number spikes synchronized by electrical recordings. We have developed new iterative methods for detecting single APs from microscopy cells, enabling all-optical detection system with high accuracy and good temporal resolution. demonstrate performance method across multiple preparations HEK-293 compare outcomes measurements ground truth detected on multi-electrode array. initially use spike-triggered average, recording, measure AP reach up 3 nm (0.9 mrad) rise time 4 ms fall about 120 ms. Based this knowledge dynamics, data analysis provide reliable spike detection, within standard deviation 11.6 (9.7% length potential) 8.5% false negative rate. The robust natural variations between be modified function any prior dynamics. Such could achieve high-throughput network activity culture help identify mechanisms linking changes transmembrane potential.