Integrating multiple omics to identify common and specific molecular changes occurring in Arabidopsis under chronic nitrate and sulfate limitations.

摘要: Plants have fundamental dependences on nitrogen and sulfur frequently to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, transcriptomic changes occurring in Arabidopsis leaves under nitrate (Low-N) sulfate (Low-S) order compare effects, determine interconnections, examine strategies of adaptation. Metabolite profiling globally revealed opposite effects Low-S Low-N carbohydrate amino acid accumulations, whilst proteomic data showed that both treatments resulted increases catabolic processes, stimulation mitochondrial cytosolic metabolism, decreases chloroplast metabolism. Lower abundances ribosomal proteins translation factors corresponded growth limitation. At transcript level, major specific effect was enhancement expression defence immunity genes. The main a decrease transcripts genes involved cell division, DNA replication, cytoskeleton, an increase autophagy consistent role target-of-rapamycin kinase control plant metabolism division Low-S. In addition, decreased several NLP transcription factors, which are master actors sensing. Finally, transcriptome proteome indicated repressed glucosinolate synthesis, exacerbated degradation. importance as buffering molecules for N S management.