Skin extracellular matrix components accelerate the regenerative potential of Lin− cells
作者： Giedrė Ramanauskaitė , Dovilė Žalalytė , Vytautas Kašėta , Aida Vaitkuvienė , Lilija Kalėdienė
DOI: 10.2478/S11535-013-0283-9
关键词: Extracellular matrix 、 Cell biology 、 Basement membrane 、 Matrix (biology) 、 Cell 、 Cell migration 、 Flow cytometry 、 Cell growth 、 Transplantation 、 Biology
摘要: Due to their unique properties, bone marrow-derived Lin− cells can be used regenerate damaged tissues, including skin. The objective of our study was determine the influence skin tissue-specific microenvironment on mouse cell proliferation and migration in vitro. Cells were analyzed for expression stem/progenitor surface markers by flow cytometry. Proliferation MACS-purified 3D cultures investigated WST-8 assay. evaluated vitro scratch results obtained show that basement membrane matrix is more effective However, type I collagen better enhances re-epithelization process, depends migratory properties. These studies are important preparing transplantation.
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