Extracellular release of antimicrobial defensins by human polymorphonuclear leukocytes.

摘要: Human polymorphonuclear leukocytes (PMN) contain three antimicrobial and cytotoxic peptides which belong to a family of mammalian granulocyte named defensins. To determine their potential availability for extracellular microbicidal or events, we quantified the release defensins after stimulation human PMN with phorbol myristate acetate opsonized zymosan. As determined by enzyme immunoassay confirmed polyacrylamide gel electrophoresis densitometry, 10(6) contained 4 5 micrograms After high concentration (1 microgram/ml), about 8% were found in media. Release correlated best azurophil granule marker beta-glucuronidase elastase poorly either specific lactoferrin cytoplasmic lactate dehydrogenase. Phagocytosis zymosan resulted less than 3% The factors responsible into media relative other proteins may include heterogeneity granules affinity cellular surfaces particles. In vivo, are most likely reach effective concentrations PMN-rich exudates (pus), confined environments phagolysosomes, intercellular clefts between targets.