Subcellular Localization of Galloylated Catechins in Tea Plants [Camellia sinensis (L.) O. Kuntze] Assessed via Immunohistochemistry.
作者： Huanhuan Xu , Ya Wang , Yana Chen , Pan Zhang , Yi Zhao
关键词: Antibody 、 Immunohistochemistry 、 Subcellular localization 、 Staining 、 Molecular biology 、 Biosynthesis 、 Camellia sinensis 、 Monoclonal antibody 、 Vacuole 、 Biochemistry 、 Biology
摘要: Galloylated catechins, as the main secondary metabolites in tea plant, including (-)-epigallocatechin-3-gallate and (-)-epicatechin-3-gallate, comprise approximately three-quarters of all plant catechins have stronger effects than non-galloylated both on product quality processing pharmacological efficacy to human beings. The subcellular localization galloylated has been primary focus studies that assess biosynthesis physiological functions. Classical histochemical staining reagents can not specifically detect catechins; thus, their remains controversial. In present study, we generated a monoclonal antibody (mAb) against which be used for by immunohistochemistry. Direct ELISA ForteBio Octet Red 96 System assay indicated mAb could recognize with high specificities affinities. addition, bud was ascertained optimal tissue freezing microtomic sections What’s more, mAbs exhibited excellent binding capability were utilised visualization them via Our findings demonstrated vacuoles sites at level.
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