Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.
作者： David M. Dohan , Joseph Choukroun , Antoine Diss , Steve L. Dohan , Anthony J.J. Dohan
DOI: 10.1016/J.TRIPLEO.2005.07.009
关键词: Fibrin 、 Platelet 、 Platelet concentrate 、 Centrifugation 、 Degranulation 、 Platelet-rich fibrin 、 Cell biology 、 Chemistry 、 Cytokine 、 Fibrin matrix 、 Biochemistry
摘要: Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features biomaterial. During PRF by centrifugation, platelets are activated their massive degranulation implies very significant cytokine release. Concentrated platelet-rich plasma cytokines have already been quantified in many technologic configurations. To carry out comparative study, therefore undertook quantify PDGF-BB, TGFβ-1, IGF-I within PPP (platelet-poor plasma) supernatant clot exudate serum. These initial analyses revealed that slow polymerization during leads intrinsic incorporation glycanic chains meshes. This result would imply PRF, unlike other be able progressively release matrix remodeling; such mechanism might explain clinically observed healing properties PRF.
elsevier.com
petrasgerve.lt 
researchgate.net 参考文章(25)
G Weibrich, W K Kleis, W Wagner, A H Loos, M Kunz-Kostomanolakis, Correlation of platelet concentration in platelet-rich plasma to the extraction method, age, sex, and platelet count of the donor. International Journal of Oral & Maxillofacial Implants. ,vol. 16, pp. 693- 699 ,(2001)
Gernot Weibrich, Wilfried K. G. Kleis, Rainer Buch, Walter E. Hitzler, Gerd Hafner, The Harvest Smart PRePTM system versus the Friadent-Schütze platelet-rich plasma kit. Clinical Oral Implants Research. ,vol. 14, pp. 233- 239 ,(2003) , 10.1034/J.1600-0501.2003.140215.X
Robert Zimmermann, Rafael Jakubietz, Michael Jakubietz, Erwin Strasser, Andreas Schlegel, Jorg Wiltfang, Reinhold Eckstein, Different preparation methods to obtain platelet components as a source of growth factors for local application. Transfusion. ,vol. 41, pp. 1217- 1224 ,(2001) , 10.1046/J.1537-2995.2001.41101217.X
Gernot Weibrich, Wilfried K.G. Kleis, Gerd Hafner, W.E. Hitzler, Wilfried Wagner, Comparison of platelet, leukocyte, and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank. Clinical Oral Implants Research. ,vol. 14, pp. 357- 362 ,(2003) , 10.1034/J.1600-0501.2003.00810.X
Alison J Butt, Sue M Firth, Robert C Baxter, The IGF axis and programmed cell death. Immunology and Cell Biology. ,vol. 77, pp. 256- 262 ,(1999) , 10.1046/J.1440-1711.1999.00822.X
Robert E Marx, Eric R Carlson, Ralph M Eichstaedt, Steven R Schimmele, James E Strauss, Karen R Georgeff, Platelet-rich plasma Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. ,vol. 85, pp. 638- 646 ,(1998) , 10.1016/S1079-2104(98)90029-4
Emmanuel Soffer, Jean Pierre Ouhayoun, Fani Anagnostou, Fibrin sealants and platelet preparations in bone and periodontal healing Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. ,vol. 95, pp. 521- 528 ,(2003) , 10.1067/MOE.2003.152
Carl-Henrik Heldin, Simultaneous induction of stimulatory and inhibitory signals by PDGF FEBS Letters. ,vol. 410, pp. 17- 21 ,(1997) , 10.1016/S0014-5793(97)00318-9
Thorsten R. Appel, Bernd Pötzsch, Jens Müller, Jens-Jörg Von Lindern, Stefaan J. Bergé, Rudolf H. Reich, Comparison of three different preparations of platelet concentrates for growth factor enrichment Clinical Oral Implants Research. ,vol. 13, pp. 522- 528 ,(2002) , 10.1034/J.1600-0501.2002.130512.X
A. Simonpieri, J. Choukroun, M.O. Girard, T. Ouaknine, D. Dohan, Implantation immédiate post-extractionnelle (IIPE) : l'intérêt du PRF® Implantodontie. ,vol. 13, pp. 177- 189 ,(2004) , 10.1016/J.IMPLAN.2004.06.004