Structural characterization of NORAD reveals a stabilizing role of spacers and two new repeat units.
作者： Toni Gabaldon , Ester Saus , Uciel Chorostecki
DOI: 10.1101/2021.03.29.437547
关键词: Sequence (medicine) 、 Structural motif 、 Cellular functions 、 Computational biology 、 RNA 、 Genomic Stability 、 Protein secondary structure 、 Function (biology) 、 Biology
摘要: Long non-coding RNAs (IncRNAs) can perform a variety of key cellular functions by interacting with proteins and other RNAs. Recent studies have shown that the function IncRNAS are largely mediated their structures. However, our structural knowledge for most is limited to sequence-based computational predictions. Non-coding RNA activated DNA damage (NORAD) an atypical IncRNA due its abundant expression high sequence conservation. NORAD regulates genomic stability microRNAs. Previous characterization has identified modular organization composed several repeat units (NRUs). These comprise protein-binding elements separated regular spacers unknown function. Here, we experimentally determine first time secondary structure using nextPARS approach. Our results suggest spacer regions provide NRUs. Furthermore, uncover two previously-unreported NRUs, core motifs conserved across Overall, these findings will help elucidate evolution NORAD.
sci-hub.st 参考文章(48)
Eugenio Mattei, Marco Pietrosanto, Fabrizio Ferrè, Manuela Helmer-Citterich, Web-Beagle: a web server for the alignment of RNA secondary structures Nucleic Acids Research. ,vol. 43, pp. 493- 497 ,(2015) , 10.1093/NAR/GKV489
A J Zaug, T R Cech, Analysis of the structure of Tetrahymena nuclear RNAs in vivo: telomerase RNA, the self-splicing rRNA intron, and U2 snRNA. RNA. ,vol. 1, pp. 363- 374 ,(1995)
André Galarneau, Stéphane Richard, The STAR RNA binding proteins GLD-1, QKI, SAM68 and SLM-2 bind bipartite RNA motifs. BMC Molecular Biology. ,vol. 10, pp. 47- 47 ,(2009) , 10.1186/1471-2199-10-47
Irina V Novikova, Scott P Hennelly, Karissa Y Sanbonmatsu, None, Structural architecture of the human long non-coding RNA, steroid receptor RNA activator Nucleic Acids Research. ,vol. 40, pp. 5034- 5051 ,(2012) , 10.1093/NAR/GKS071
Sylvain Maenner, Magali Blaud, Laetitia Fouillen, Anne Savoye, Virginie Marchand, Agnès Dubois, Sarah Sanglier-Cianférani, Alain Van Dorsselaer, Philippe Clerc, Philip Avner, Athanase Visvikis, Christiane Branlant, 2-D Structure of the A Region of Xist RNA and Its Implication for PRC2 Association PLoS Biology. ,vol. 8, pp. e1000276- 1000276-16 ,(2010) , 10.1371/JOURNAL.PBIO.1000276
Rick Russell, RNA misfolding and the action of chaperones Frontiers in Bioscience. ,vol. 13, pp. 1- 20 ,(2008) , 10.2741/2557
Qiong Lin, Stephen J. Taylor, David Shalloway, Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. Journal of Biological Chemistry. ,vol. 272, pp. 27274- 27280 ,(1997) , 10.1074/JBC.272.43.27274
Zasha Weinberg, Ronald R Breaker, R2R - software to speed the depiction of aesthetic consensus RNA secondary structures BMC Bioinformatics. ,vol. 12, pp. 3- 3 ,(2011) , 10.1186/1471-2105-12-3
Srinivas Somarowthu, Michal Legiewicz, Isabel Chillón, Marco Marcia, Fei Liu, Anna Marie Pyle, HOTAIR Forms an Intricate and Modular Secondary Structure Molecular Cell. ,vol. 58, pp. 353- 361 ,(2015) , 10.1016/J.MOLCEL.2015.03.006
Alessandro Fatica, Irene Bozzoni, Long non-coding RNAs: new players in cell differentiation and development Nature Reviews Genetics. ,vol. 15, pp. 7- 21 ,(2014) , 10.1038/NRG3606