Specific association of T-2 toxin with mammalian cells

摘要: The binding of radiolabeled T-2 toxin to a mammalian cell line derived from Chinese hamster ovary (CHO) was studied. bound to, or taken up by, cells in time-, temperature- and concentration-dependent manner. saturable, high affinity (Kd approximately 0.1 1 nM), reversible at 37 degrees (half-time 2 hr), specific. kinetics T-2-cell association the rate toxin-induced inhibition protein synthesis closely paralleled one another. Likewise, concentration-response for isotherm were similar. A synthetically epimer less tightly cells, but apparently same site as authentic T-2. also potent inducing synthesis. Two other trichothecene toxins, more toxic than T-2, blocked labeled manner reflective their inhibitory potencies. We conclude that we defined is an accurate measure responsible CHO cells. data suggested that, equilibrium, interaction with not static, sum continuous uptake release process.