Bacterial and fungal activities in soil: Separation of bacteria and fungi by a rapid fractionated centrifugation technique
作者： Agnete Fægri , Vigdis Lid Torsvik , J. GoksÖyr
DOI: 10.1016/0038-0717(77)90045-1
关键词: Microscopic count 、 Biology 、 Soil water 、 Homogenization (biology) 、 Chromatography 、 Centrifugation 、 Hypha 、 Botany 、 Respiration 、 Bacteria 、 Soil science 、 Microbiology
摘要: With three natural soils from Western and Central Norway it has been shown that by homogenization and fractionated centrifugations, two fractions could be obtained, one containing 50–80% of the bacteria, and the other all the fungi together with soil debris and the rest of the bacteria. When the separation was carried out in the cold and lasting less than 3–4 h, the fractions if used immediately had constant respiratory rates for 1–2 h. The sum of the rates agreed well with that of an unfractionated soil homogenate, making it possible to …
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sci-hub.se 参考文章(13)
S. Hinneri, M. Sonesson, A. K. Veum, Soils of Fennoscandian IBP Tundra Ecosystems. Ecological studies. ,vol. 16, pp. 31- 40 ,(1975) , 10.1007/978-3-642-80937-8_2
P.F. Scholander, ANALYZER FOR ACCURATE ESTIMATION OF RESPIRATORY GASES IN ONE-HALF CUBIC CENTIMETER SAMPLES Journal of Biological Chemistry. ,vol. 167, pp. 235- 250 ,(1947) , 10.1016/S0021-9258(17)35161-X
J. Goksøyr, Decomposition, Microbiology, and Ecosystem Analysis Springer Berlin Heidelberg. pp. 230- 238 ,(1975) , 10.1007/978-3-642-80937-8_26
Jacques Pochon, Manuel technique d'analyse microbiologique du sol Masson. ,(1954)
H. Lyr, Werner Gillwald, Holzzerstörung durch Pilze Akademie-Verlag. ,(1963)
L. E. Casida, Microorganisms in Unamended Soil as Observed by Various Forms of Microscopy and Staining 1 Applied Microbiology. ,vol. 21, pp. 1040- 1045 ,(1971) , 10.1128/AM.21.6.1040-1045.1971
D. L. Balkwill, D. P. Labeda, L. E. Casida Jr., Simplified procedures for releasing and concentrating microorganisms from soil for transmission electron microscopy viewing as thin-sectioned and frozen-etched preparations Canadian Journal of Microbiology. ,vol. 21, pp. 252- 262 ,(1975) , 10.1139/M75-036
H. G. THORNTON, ON THE DEVELOPMENT OF A STANDARDISED AGAR MEDIUM FOR COUNTING SOIL BACTERIA, WITH ESPECIAL REGARD TO THE REPRESSION OF SPREADING COLONIES Annals of Applied Biology. ,vol. 9, pp. 241- 274 ,(1922) , 10.1111/J.1744-7348.1922.TB05958.X
I. C. Gunsalus, Roger Y. Stanier, The bacteria: a treatise on structure and function : Academic Press. ,(1960)
M. Sonesson, F. E. Wielgolaski, P. Kallio, Description of Fennoscandian Tundra Ecosystems Ecological studies. ,vol. 16, pp. 3- 28 ,(1975) , 10.1007/978-3-642-80937-8_1