Cell cycle kinetics following UVA irradiation in comparison to UVB and UVC irradiation.
作者： Annemarie Laat , Marloes Tilburg , Jan C. Leun , Willem A. Vloten , Frank R. Gruijl
DOI: 10.1111/J.1751-1097.1996.TB03075.X
关键词: Bromodeoxyuridine 、 DNA synthesis 、 Kinetics 、 Carcinogen 、 Cell Cycle Kinetics 、 DNA damage 、 Molecular biology 、 Photochemistry 、 Chemistry 、 DNA 、 Cell cycle
摘要: — There is limited information about the carcinogenic effect of longwave ultraviolet radiation (UVA: 315-400 nm). In particular very little known relevant genotoxic damage caused by physiological doses UVA radiation. A general response cells to DNA a delay or arrest cell cycle. Conversely, such cellular responses after irradiation would indicate significant damage. The aim this study compare cycle kinetics human fibroblasts UVC (190-280 nm radiation), UVB (280-315 radiation) and irradiation. Changes in were assessed bivariate flow cytometric analysis synthesis content. After UVC, suppression was seen bromodeoxyuridine (BrdU) incorporation at all stages S phase. magnitude appeared dose dependent. Maximum reached 5-7 h exposure directly exposure, normal levels 25 7 exposure. lowered BrdU uptake corresponded with lengthening No dramatic changes percentages G1, G2/M various UV irradiations. Apparently, irradiation, like can temporarily inhibit synthesis, which indicative
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