Activation of polyphenol oxidase by polyamines.

摘要: Latent polyphenol oxidase was extracted and partially purified from grape cell suspension cultures. The enzyme shown to be activated by polyamines. Activation of the increased with increasing polyamine concentrations half-maximal activation in order 8mM. Kinetic parameters, Km Vm, were also calculated for latent enzymes. activating effect polyamines studied at different pH values. Optimum 4.5 However, highest degree obtained 5. caused a higher sensitivity temperature. ability activate may suggest limited conformational change.