Isolation of Microtubule Protein from Cultured Mouse Neuroblastoma Cells
作者： J. B. Olmsted , K. Carlson , R. Klebe , F. Ruddle , J. Rosenbaum
关键词: Amino acid 、 Molecular biology 、 Microtubule 、 Centrifugation 、 Mitosis 、 Colchicine 、 Organoid 、 Plasma protein binding 、 Biology 、 Neuroblastoma
摘要: The addition of vinblastine to high-speed supernatants derived from homogenates cultured mouse neuroblastoma cells results in the formation a precipitate which has been characterized as microtubule protein by following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. method therefore permits rapid isolation crude cells.
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