3'-(p-azidobenzamido)taxol photolabels the N-terminal 31 amino acids of beta-tubulin.
作者： S. Rao , N.E. Krauss , J.M. Heerding , C.S. Swindell , I. Ringel
DOI: 10.1016/S0021-9258(17)41836-9
关键词: Binding site 、 Tubulin 、 Polyacrylamide gel electrophoresis 、 Mechanism of action 、 Fluorescence spectrometry 、 Amino acid 、 Cleavage (embryo) 、 Stereochemistry 、 Microtubule 、 Biology
摘要: … undigested photolabeled &tubulin and a 12%polyacrylamide gelplus 6 M urea was used for … P-tubulin was electroeluted from polyacrylamide gels and digested with formic acid. Formic …
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