Tea extract render probiotic Lactobacillus helveticus more resistant to oxygen exposure through lipid modification mechanism

摘要: Exposure to oxygen can cause a decrease in growth rates or complete inhibition of oxygen-sensitive probiotic bacteria. A recent study our laboratory demonstrated that the an strain, Lactobacillus helveticus, was stimulated, under aerobic conditions, when culture medium enriched with green tea extracts (GTE). However, information on mechanism by which GTE influenced growth, presence oxygen, strain is limited. In present work, effects concentrations (0 2000 μg/mL) and exposure maximal populations L. helveticus R0052 cells bacterial lipids were evaluated using viable counts, infrared spectroscopy gas chromatography analyses. Supplementation 0 500 μg/mL did not have effect reached microaerophilic conditions lipid structure composition. at GTE, high population levels concomitant increase order important changes fatty acid composition lipids. Interactions between components shown spectroscopic results. Moreover, adapted changing their To best knowledge, this work first establish relationship cell's stimulation strain.