Automated oxyhemoglobin dissociation curve construction to assess sickle cell anemia therapy.

摘要: Oxyhemoglobin dissociation curves measure the most important function of red blood cells - affinity for oxygen and its delivery to tissues. This may be deranged in sickle cell anemia some other hemoglobinopathies. An automated oxyhemoglobin curve analyzer constructed 55 patients with hemoglobinopathies 24 control subjects while maintaining constant temperature pH. Sigmoid were converted rectilinear ones using Hill equation. Oxygen was assessed by calculation P50 (the PO2 at which hemoglobin is half saturated). Results revealed separation plots according phenotype but wide variability. Mean fetal greatest, whereas that least. Other hemoglobins intermediate. A positive correlation between decreased carboxyhemoglobin confirmed increased diphosphoglycerate cells. are less sensitive discriminators than traditional sigmoid offer no particular advantage. Serial studies a subset three treated conservatively suggest useful assessment effectiveness newer therapies after refinement method larger populations.