FTIR microscopic imaging of carcinoma tissue section with 4× and 15× objectives: Practical considerations

摘要: The advent of FTIR microscopic spectrometers with focal plane array detectors enabled rapid image acquisition diffraction limited lateral resolution. field view depends on the magnification and detector size. images large samples can be collected in so called mosaic mode by stitching individual together. If is composed hundreds images, total time data size will increase considerably. One computational two optical options are compared to reduce both First, 2× expansion optic increases measured sample area fourfold. Second, using a 4× objective instead standard 15× covered single factor 11. Third, pixel binning averages neighboring pixels at expense All demonstrated case study thin section laryngeal carcinoma encompassing normal tissue, inflammation, connective dysplasia, blood. Data analysis described toolbox hyperSpec operating under R environment complemented parallel computing functions. A classification model that was trained low range from 1200 1800 cm−1 gave similar results for higher data. Restrictions occurred features smaller than biomarkers below due signal attenuation lenses. imaging strategies other groups emerging use quantum cascade lasers IR discussed.