Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes
作者： MJ Aman , T Tretter , I Eisenbeis , G Bug , T Decker
DOI: 10.1182/BLOOD.V87.11.4731.BLOODJOURNAL87114731
关键词: Cytokine 、 Monocyte 、 Molecular biology 、 CD3 、 Peripheral blood mononuclear cell 、 Interleukin 10 、 CD28 、 Immunology 、 Lipopolysaccharide 、 Biology 、 Alpha interferon
摘要: In the present study, we investigated effect of interferon-alpha (IFN-alpha) on expression interleukin-10 (IL-10) mRNA and protein synthesis in human monocytes CD4+ T cells. mononuclear cells, IFN-alpha induced IL-10 further enhanced lipopolysaccharide (LPS)-stimulated expression. purified monocytes, a strong by LPS was not IFN-alpha. highly IFN- alpha upregulated upon activation with phytohemagglutinin phorbol myristate acetate. an dependent costimulation LPS. Maximal stimulation seen after prolonged incubation periods 48 to 96 hours, whereas IFN-gamma reduced production early period. Similar effects were observed cells activated CD3 CD28 monoclonal antibodies. Addition caused increase culture supernatants T-helper more than 100% hours incubation. contrast, other cytokines, including IL-4, had no influence secretion stimulated serum samples IFN-alpha-treated individuals, failed detect cytokine treatment levels, confirming requirement additional activating signals for IFN-alpha-mediated synthesis. conclusion, enhances late induction IL- 10, which physiologically occurs Biologically, this might enhance negative-feedback mechanism ascribed IL-10, limits inflammatory reactions.
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