FBW7 Regulates the Autophagy Signal in Mesangial Cells Induced by High Glucose
作者： Chenlin Gao , Fang Fan , Jiao Chen , Yang Long , Shi Tang
DOI: 10.1155/2019/6061594
关键词: Proteasome 、 Ubiquitin ligase 、 Proinflammatory cytokine 、 Inflammation 、 Downregulation and upregulation 、 Cell biology 、 Autophagy 、 PI3K/AKT/mTOR pathway 、 Signal transduction 、 Chemistry
摘要: Aims. Abnormal regulation of autophagy participates in the development diabetic nephropathy. mTOR is most common negative regulator signaling pathway. FBW7 constitutes SCF (Skp1–Cullin1–F-box protein) recognition subunit E3 ubiquitin ligase, and a substrate that can be modified by ubiquitination degraded via proteasomes. In this study, we explored relationship between examined effects on occurrence nephropathy vitro. Materials Methods. We cultured mesangial cells induced high glucose vitro used rapamycin as specific inhibitor, performed gene overexpression, detected expression signal inflammatory factors WB, ELISA, RT-PCR, immunofluorescence. Results. High downregulate activate signal, which leads to diminished renal cells, well cytokines fibrotic factors. RAPA, specifically inhibitor mTOR, decrease inhibiting mTOR. Moreover, overexpression increase signal; at same time, were decreased cells. Conclusions. was glucose, ameliorate inflammation fibrosis.
hindawi.com参考文章(42)
Daniel F. Egan, Matthew G.H. Chun, Mitchell Vamos, Haixia Zou, Juan Rong, Chad J. Miller, Hua Jane Lou, Dhanya Raveendra-Panickar, Chih-Cheng Yang, Douglas J. Sheffler, Peter Teriete, John M. Asara, Benjamin E. Turk, Nicholas D.P. Cosford, Reuben J. Shaw, Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates Molecular Cell. ,vol. 59, pp. 285- 297 ,(2015) , 10.1016/J.MOLCEL.2015.05.031
Jie Liu, John Weaver, Xinchun Jin, Yuan Zhang, Ji Xu, Ke J. Liu, Weiping Li, Wenlan Liu, Nitric Oxide Interacts with Caveolin-1 to Facilitate Autophagy-Lysosome-Mediated Claudin-5 Degradation in Oxygen-Glucose Deprivation-Treated Endothelial Cells Molecular Neurobiology. ,vol. 53, pp. 5935- 5947 ,(2016) , 10.1007/S12035-015-9504-8
Hiroyuki Okazaki, Naoya Matsunaga, Takashi Fujioka, Fumiyasu Okazaki, Yui Akagawa, Yuuya Tsurudome, Mayumi Ono, Michihiko Kuwano, Satoru Koyanagi, Shigehiro Ohdo, Circadian Regulation of mTOR by the Ubiquitin Pathway in Renal Cell Carcinoma Cancer Research. ,vol. 74, pp. 543- 551 ,(2014) , 10.1158/0008-5472.CAN-12-3241
Sagar Ramesh Darvekar, Julianne Elvenes, Hanne Britt Brenne, Terje Johansen, Eva Sjøttem, None, SPBP Is a Sulforaphane Induced Transcriptional Coactivator of NRF2 Regulating Expression of the Autophagy Receptor p62/SQSTM1 PLoS ONE. ,vol. 9, pp. e85262- ,(2014) , 10.1371/JOURNAL.PONE.0085262
V Kumar, R Palermo, C Talora, A F Campese, S Checquolo, D Bellavia, L Tottone, G Testa, E Miele, S Indraccolo, A Amadori, E Ferretti, A Gulino, A Vacca, I Screpanti, Notch and NF-kB signaling pathways regulate miR-223/FBXW7 axis in T-cell acute lymphoblastic leukemia Leukemia. ,vol. 28, pp. 2324- 2335 ,(2014) , 10.1038/LEU.2014.133
Chenlin Gao, Keri Aqie, Jianhua Zhu, Guo Chen, Ling Xu, Lan Jiang, Yong Xu, MG132 Ameliorates Kidney Lesions by Inhibiting the Degradation of Smad7 in Streptozotocin-Induced Diabetic Nephropathy Experimental Diabetes Research. ,vol. 2014, pp. 918396- 918396 ,(2014) , 10.1155/2014/918396
Munehiro Kitada, Ai Takeda, Takako Nagai, Hiroki Ito, Keizo Kanasaki, Daisuke Koya, Dietary Restriction Ameliorates Diabetic Nephropathy through Anti-Inflammatory Effects and Regulation of the Autophagy via Restoration of Sirt1 in Diabetic Wistar Fatty (fa/fa) Rats: A Model of Type 2 Diabetes Experimental Diabetes Research. ,vol. 2011, pp. 908185- 908185 ,(2011) , 10.1155/2011/908185
Li Fang, Yang Zhou, Hongdi Cao, Ping Wen, Lei Jiang, Weichun He, Chunsun Dai, Junwei Yang, Autophagy Attenuates Diabetic Glomerular Damage through Protection of Hyperglycemia-Induced Podocyte Injury PLoS ONE. ,vol. 8, pp. e60546- ,(2013) , 10.1371/JOURNAL.PONE.0060546
Jeremy S. Leventhal, John C. He, Michael J. Ross, Autophagy and Immune Response in Kidneys Seminars in Nephrology. ,vol. 34, pp. 53- 61 ,(2014) , 10.1016/J.SEMNEPHROL.2013.11.008
Shunrong Ji, Yi Qin, Si Shi, Xiangyuan Liu, Hongli Hu, Hu Zhou, Jing Gao, Bo Zhang, Wenyan Xu, Jiang Liu, Dingkong Liang, Liang Liu, Chen Liu, Jiang Long, Haijun Zhou, Paul J Chiao, Jin Xu, Quanxing Ni, Daming Gao, Xianjun Yu, ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer Cell Research. ,vol. 25, pp. 561- 573 ,(2015) , 10.1038/CR.2015.30