Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells.
作者： Carolina Gonzales da Silva , Carlos Frederico Martins , Heidi Christina Bessler , Álvaro Moraes da Fonseca Neto , Tereza Cristina Cardoso
DOI: 10.1111/RDA.13360
关键词: Embryo culture 、 Gene expression 、 Trichostatin A 、 Methylation 、 Andrology 、 Embryo 、 DNA methylation 、 Chemistry 、 Cellular differentiation 、 Mesenchymal stem cell
摘要: The aim of this work was to investigate the methylation and hydroxymethylation status mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) fibroblasts (FIB-control) verify effect trichostatin A (TSA) on gene expression development cloned bovine embryos produced using these cells. Characterization MSC two animals (BOV1 BOV2) performed by flow cytometry, immunophenotyping analysis cellular differentiation genes expression. were used in nuclear transfer absence or presence 50 nM TSA for 20 hr embryo culture. Expression HDAC1, HDAC3 KAT2A measured qRT-PCR. Methylation results showed difference between animals, with BOV2 demonstrating lower rate than BOV1. Meanwhile, MSC-AF less hydroxymethylated both animals. 44.92 ± 8.88% blastocysts when exposed similar MSC-AT also treated (37.96 15.80%). However, FIB compared MSC, as found BOV1, use not sufficient increase production. expressed TSA, higher all FIB. methylated more combination incubation can induce consequently improve
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