Vaccination against Streptococcus pneumoniae Using Truncated Derivatives of Polyhistidine Triad Protein D

摘要: Polyhistidine triad protein D (PhtD) has been described as a promising vaccine candidate for use against Streptococcus pneumoniae, but there lack of examination its structure and which region(s) the are targeted by protective immune responses. In this study, we purified recombinant truncated derivatives PhtD examined their secondary structural composition, well capacity to bind antibodies from polyclonal murine serum generated full length protein. This allowed identification particularly immunogenic fragment PhtD, was also characterised. The were tested antigens in mouse models pneumococcal sepsis colonisation, using alum E. coli heat labile toxin B subunit respectively adjuvants. These experiments revealed that whilst region identified may be protect sepsis, ineffective at conferring significant immunity. results potential used novel vaccines, currently being clinical trials.