Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant of Alcaligenes eutrophus
作者： Alexander Steinb�chel , Ursula Pieper
DOI: 10.1007/BF00174193
关键词: Bacteria 、 Fructose 、 3-Hydroxybutyric Acid 、 Copolyester 、 Chemistry 、 Gluconic acid 、 Mutant 、 Biochemistry 、 Amino acid 、 Metabolism
摘要: Alcaligenes eutrophus mutant strain R3, which is a spontaneous revertant to prototrophy of an isoleucine-auxotrophic the wild-type H16, accumulated copolyester consisting 3-hydroxybutyric acid (3HB) as main constituent and 3-hydroxyvaleric (3HV), i.e. poly(3HB-co-3HV), only other from various single unrelated substrates, were provided in excess, after nutrient essential for growth was depleted medium. Poly(SHB-co-3HV) produced fructose, gluconate, succinate, acetate or lactate during cell starvation nitrogen, sulphur magnesium source. Although 3HV amounted 8 mol% constituents polyester, this study provides general rationale construction utilization mutants poly(3HB)-accumulating bacteria that are altered metabolism branched-chain amino acids production poly(3HB-co-3HV) substrates.
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