Effect of allogeneic umbilical cord mesenchymal stem cell transplantation in a rat model of hepatic cirrhosis
作者： Yang Lihua , Wang Yun , Wang Xiaohua , Liu Yanqing
DOI: 10.1016/S0254-6272(15)30010-8
关键词: Andrology 、 Transplantation 、 Cirrhosis 、 Venous blood 、 Albumin 、 Medicine 、 In vivo 、 Liver function 、 Umbilical cord 、 Mesenchymal stem cell 、 Immunology
摘要: Abstract Objective To determine the effects of human umbilical cord mesenchymal stem cell (UCMSC) transplantation, alone or in combination with tanshinone IIA (Tan IIA) on hepatic cirrhosis rats. Methods A rat model was established. Rats were divided into control, UCMSC, and UCSMC plus Tan groups. UCMSC group injected via tail vein 0.2 mL DiI-labeled suspension. Intraperitoneal injections (20 mg/kg) started day transplantation group, continued for 7 consecutive days thereafter. sacrificed 1 day, 3 days, month, months after numbers UCMSCs colonizing liver determined. Albumin (ALB) alanine aminotransferase (ALT) levels measured venous blood, mRNA protein expression ALB cytokeratin (CK)-18 tissues determined by reverse transcription-polymerase chain reaction western blotting, respectively. Results Serum ALT significantly lower serum higher rats compared control ( P Conclusion Human transplanted can grow differentiate hepatocyte-like cells resulting improved function vivo. further influenced outcomes.
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