High-Resolution Magic Angle Spinning (HR-MAS) NMR-Based Fingerprints Determination in the Medicinal Plant Berberis laurina

摘要: Berberis laurina (Berberidaceae) is a well-known medicinal plant used in traditional medicine since ancient times; however, it scarcely studied to large-scale fingerprint. This work presents broad-range fingerprints determination through high-resolution magical angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy, well-established flexible analytical method and one of most powerful "omics" platforms. It had been intended describe large range chemical compositions all parts. Beyond that, HR-MAS NMR allowed the direct investigation botanical material (leaves, stems, roots) their natural, unaltered states, preventing molecular changes. The study revealed 17 metabolites, including caffeic acid, berberine, remarkable alkaloid from genus L. metabolic pattern changes leaves course time were found be seasonally dependent, probably due variability seasonal environmental trends. metabolites overview great importance understanding (bio)chemistry mediating survival influenceable by interacting means. Moreover, will helpful purposes, health sciences, crop evaluations, genetic biotechnological research.