High-Throughput Technologies: DNA and RNA Sequencing Strategies and Potential

作者: Thomas Rio Frio

DOI: 10.1007/978-3-319-22189-2_5

关键词: Small RNAModel organismExomeGenomicsGenomeEpigeneticsBiologyTranscriptomeComputational biologyRNA

摘要: Next-generation sequencing (NGS) has revolutionized the field of genomics since release first NGS sequencer in 2005. For last 10 years, second generation machines reached tremendous throughput and accuracy that enables researchers to conduct large-scale genomic projects at ever-decreasing cost time. It is now commonplace sequence entire genome individuals. This ushering a new era medicine, allowing characterizing patients tumor cells uncover disease-causing mutations, information can be used inform about best therapeutic options. High-throughput complete experiments human model organisms for genome, exome, transcriptome, small RNA analyses, localization protein binding, epigenetics marks. aims become gold standard scientific research as well clinical genetic testing.

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