iTRAQ Protein Profile Analysis of Arabidopsis Roots Reveals New Aspects Critical for Iron Homeostasis

摘要: Iron (Fe) deficiency is a major constraint for plant growth and affects the quality of edible parts. To investigate mechanisms underlying Fe homeostasis in plants, deficiency-induced changes protein profile Arabidopsis (Arabidopsis thaliana) roots were comprehensively analyzed using iTRAQ (Isobaric Tag Relative Absolute Quantification) differential liquid chromatography-tandem mass spectrometry on LTQ-Orbitrap with high-energy collision dissociation. A total 4,454 proteins identified false discovery rate less than 1.1%, 2,882 reliably quantified. subset 101 was differentially expressed upon deficiency. The profiles show low congruency previously reported alterations transcript levels, indicating posttranscriptional changes, provide complementary information processes. abundance involved synthesis/regeneration S-adenosylmethionine, phenylpropanoid pathway, response to oxidative stress, respiration highly increased by Using Fe-responsive as bait, genome-wide fishing partners predictable or confirmed interologs revealed that RNA processing ribonucleoprotein complex assembly may represent critical processes contribute regulation root responses deficiency, possibly biasing translation efficiency.