Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-α Resistance.
作者： Martin J. Deymier , Zachary Ende , Angharad E. Fenton-May , Dario A. Dilernia , William Kilembe
DOI: 10.1371/JOURNAL.PPAT.1005154
关键词: Transmission (mechanics) 、 Peripheral blood mononuclear cell 、 Immune system 、 Infectivity 、 Alpha interferon 、 Antibody 、 Viral replication 、 Biology 、 Virology 、 Genetics 、 Viral quasispecies
摘要: Heterosexual transmission of HIV-1 is characterized by a genetic bottleneck that selects single viral variant, the transmitted/founder (TF), during most events. To assess characteristics influencing transmission, we sequenced 167 near full-length genomes and generated 40 infectious molecular clones (IMC) including TF variants multiple non-transmitted (NT) subtype C from six linked heterosexual pairs time transmission. Consensus-like sensitive to donor antibodies were selected for in these pairs. However, did not demonstrate increased fitness terms particle infectivity or replicative capacity activated peripheral blood mononuclear cells (PBMC) monocyte-derived dendritic (MDDC). In addition, resistance variant antiviral effects interferon-α (IFN-α) was significantly different same pair. Thus neither vitro nor IFN-α discriminated potential viruses quasispecies chronically infected individuals. our findings support hypothesis within-host evolution response adaptive immune responses reduces potential.
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