Expression of leukocyte adherence-related glycoproteins during differentiation of HL-60 promyelocytic leukemia cells.

摘要: We used the HL-60 human promyelocytic leukemia cell line to analyze surface expression of a family adherence-related leukocyte antigens during myeloid differentiation. These are composed discrete alpha subunits, designated L, M, and X, that each noncovalently associated with common beta subunit. Monoclonal antibodies directed against individual subunits served as markers in both indirect immunofluorescence studies immunoprecipitations from cells differentiated preferentially towards mature granulocytes (DMSO, retinoic acid) or monocyte/macrophages (PMA, vitamin D3). In undifferentiated cells, L X were constitutively expressed, whereas M subunit was not. Differentiation along granulocytic pathway DMSO resulted marked increase minimal increases X. The phenotypic these on DMSO-treated closely resembled normal circulating PMN. monocyte/macrophage when using PMA D3 major expression, well M. changes phenotype characteristic present monocyte-derived macrophages. Triggering caused no stimulation DMSO-differentiated produced more than 1.5-fold enhancement PMN increased fourfold twofold. Stimulation change any three populations. results indicate this glycoprotein can be selectively regulated vitro differentiation line. Second, possessed an intracellular pool but not could translocated surface. Thus, despite structural functional relationships among family, they undergo disparate regulation