Immunocytochemical localization of the glucocorticoid receptor in steroid-sensitive and -resistant human leukemic cells.

摘要: Because of their lympholytic action, glucocorticoids are included in most therapeutic regimens for the treatment lymphomas and leukemias. The presence functional glucocorticoid receptor may be predictive response to hormonal therapy these diseases. We have developed an immunocytochemical procedure human (GR) order assess its level subcellular distribution a well-studied system childhood lymphoblastic leukemia cells (CEM), where sensitive resistant subclones been established. Several fixation cell permeabilization protocols were compared. reproducible one light microscopy was prefixation Bouin's solution followed by cytocentrifugation. Using various polyclonal monoclonal antibodies, GR consistently localized predominantly cytoplasm absence steroid. compared localization following glucocorticoid-sensitive clone CEM C7 with (4R4, 3R43, ICR27). Upon incubation glucocorticoid, increase nuclear staining clearly observed steroid-sensitive cells. Although lines contain immunoreactive GR, they failed show translocation treatment. This outlines importance distinguish between leukemic Whether this test could used prospectively help select leukemias can now examined.