Mutations generated in human immunodeficiency virus type 1 long terminal repeat during vertical transmission correlate with viral gene expression

摘要: Abstract We determined the effect of mutations generated in HIV-1 LTR on viral gene expression six mother–infant pairs following vertical transmission. show that functional domains critical for function, promoter (TATAA), enhancers (three SpI and two NFκB sites), modulatory region (two AP-I sites, NFAT, one NF-IL6 site, Ets-1, USF-1) TAR were generally conserved among pairs, although we observed several patient pair specific these important domains. then activity our sequences by measuring CAT expression, which was driven LTRs found most derived from 6 functional. However, transcription factor binding including TATAA, SpI, NFκB, AP-I, NF-IL6, USF-1 resulted reduced expression. Taken together, conservation during transmission supports notion a is replication pathogenesis course infection correlated with